Abstract
We show herein that CNT–cell complexes are formed in the presence of a magnetic field.The complexes were analyzed by flow cytometry as a quantitative method formonitoring the physical interactions between CNTs and cells. We observed an increasein side scattering signals, where the amplitude was proportional to the amountof CNTs that are associated with cells. Even after the formation of CNT–cellcomplexes, cell viability was not significantly decreased. The association betweenCNTs and cells was strong enough to be used for manipulating the complexes andthereby conducting cell separation with magnetic force. In addition, the CNT–cellcomplexes were also utilized to facilitate electroporation. We observed a time constantfrom CNT–cell complexes but not from cells alone, indicating a high level ofpore formation in cell membranes. Experimentally, we achieved the expression ofenhanced green fluorescence protein by using a low electroporation voltage after theformation of CNT–cell complexes. These results suggest that higher transfectionefficiency, lower electroporation voltage, and miniaturized setup dimension ofelectroporation may be accomplished through the CNT strategy outlined herein.
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