Abstract
Objective To investigate the interaction and cytotoxicity of the photosensitizer mTHPC [5, 10, 15, 20-tetrakis (3-hydroxyphenyl) chlorine]and the complex human serum albumin (HSA)-mTHPC in Jurkat cells. Methods Jurkat cells were incubated with different concentrations of mTHPC and HSA-mTHPC for4, 24 and 48 h, followed by illumination with 652 nm for 10 min with 10 mW/cm2. The cellular interaction of free mTHPC and HSA-mTHPC was measured by using flow cytometry. The cytotoxicity was tested using standard toxicity assays (the WST-l-assay for the cell viability, the LDH-assay for the membrane integrity, and the BrdU-assay for the proliferation). Results After incubation with 1.0 mg/L mTHPC for 4 and 24 h, 98.07% and 99.07% of the cells were positive for the photosensitizer. The interaction of HSA without drug with the cells was low. The uptake of mTHPC that was compounded to HSA was as high as of free mTHPC (4 h: 98.13%; 24 h: 99.19%). After illumination, the cell viability was decreased to 13.27%, 5.46% and 4.99% after incubation with 1.0 mg/L mTHPC 4, 24 and 48 h, respectively. The LDH leakage was increased up to 100% even at low concentrations of mTHPC. The cell proliferation was also decreased in a concentration-and time-dependent manner. After incubation with 1.0 mg/L mTHPC for 48 h and subsequent illumination, only marginal proliferation could be detected (0.62% ). Conclusion Both mTHPC and HSA-mTHPC are photosensitizer formulations with potent anti-tumor effects, which can enter tumor cells effectively and cause cell death. HSA nanoparticles can be used as mTHPC carriers. Key words: Photodynamic therapy; Photosensitizer; mTHPC; Jurkat cells
Published Version
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