INTENSITY OF LIPID PEROXIDAL OXIDATION PROCESSES IN RATS' BLOOD AT CONTINUOUS CADMIUM AND LEAD LOAD

Abstract

Lead and Cadmium are attributed to thiol poisons due to their ability to bind to SH groups of proteins. The aim of the study was to study the effect of Cadmium and Lead salts on the intensity of lipid peroxidation processes in the blood of rats. The experiments were performed on male rats of the Wistar line, weighing 200–220 g, from which 4 groups of animals were formed: control and 3 experimental groups. Control rats were administered drinking water through a metal probe in a volume equivalent to the volume of aqueous solution of Cd2 + and Pb2 + salts. The animals of the first experimental group were administered a 0.029% aqueous solution of cadmium chloride at a dose of 4.0 mg / kg. Animals of the second experimental group were administered a 16.6% aqueous solution of lead acetate at a dose of 200 mg / kg. Animals of the third experimental group were administered 16.6% aqueous lead acetate solution at a dose of 100 mg / kg and 0.029% aqueous cadmium chloride solution at a dose of 2.0 mg / kg.
 The administration of cadmium chloride and lead acetate to the body leads to the accumulation in the blood of rats of the experimental groups of both intermediate and final products of lipid peroxidation. A significant increase in the level of lipid peroxidation products was observed in the blood of rats of all three study groups from the first day of the experiment. With cadmium and lead combined, rats were found to have the highest levels of diene conjugates and TBA-active products compared to the first and second experimental groups. On the 28th day of the experiment, the level of lipid peroxidation oxidation products in the blood of rats of the third experimental group increased by 76.1%, and the level of TBA-active products - by 38.4% relative to the control group. These changes in the level of lipid peroxidation products indicate an increase in the intensity of radical formation processes. Peroxide oxidation forms, at almost all stages of its course, a number of products that result from the interaction of free radicals with each other and with biological macromolecules.
 The investigations made it possible to reveal the pathogenesis of Cadmium and Lead toxic effects on the rat organism more deeply and to use these data in the development of antidote for cadmium-lead intoxication.