DYNAMICS OF INDICATORS OF THE BLOOD ANTIOXIDANT SYSTEM OF RATS UNDER CONDITIONS OF OXIDATIVE STRESS AND THE ACTION OF THE LIPOSOMAL PREPARATION

Abstract

The aim of the study – to study the effect of the liposomal drug «Butaintersil» on theantioxidant status of rats under conditions of oxidative stressMaterials and methods. Modeling of oxidative stress was performed on sexually maturemale Wistar rats with a body weight of 180-200 g. In the blood of rats, changes inglutathione peroxidase activity and the level of reduced glutathione, as well as the levels oflipid peroxidation products, lipid hydroperoxide, and TBC-active products, were studied.Results. Intramuscular injection of a 50 % oily solution of tetrachloromethane at a doseof 0.25 ml/100 g of the animal’s body weight led to a change in the activity of the glutathionesystem of antioxidant protection: a decrease in the activity of glutathione peroxidaseand the level of reduced glutathione. At the same time, an increase in lipid peroxidationprocesses, namely an increase in the level of lipid hydroperoxides and TBC-activeproducts during the entire research period was observed in the intoxicated animals. In itsturn, with the additional administration of the liposomal drug «Butaintersil» to animalsunder conditions of tetrachloromethane intoxication, strengthening of the antioxidantstatus of the body of experimental animals was established. Thus, on the 14th day of the experiment, the activity of glutathione peroxidase and the level of reduced glutathionewere the highest in the blood of rats of the second experimental group, where comparedto intoxicated animals, they were 2.1 and 2.37 times higher, respectively. Also, underthe influence of the studied liposomal drug, changes in the levels of lipid peroxidationproducts in the blood of rats of the second experimental group were detected, namely,a decrease in the level of lipid hydroperoxides and TBC-active products in their blood.Conclusions. The obtained results indicate the corrective effect of Butaintersil on theparameters of the antioxidant protection of the body of rats after their intoxication withtetrachloromethane.