Intensification of growth of Schizophyllum commune using laser irradiation

Abstract

The growth processes of the basidiomycete fungus Schizophyllum сommune under the action of laser irradiation have been studied. To determine the growth rate of strains S.c.-01, S.c.-02, S.c.-03 of the fungus S. commune mycelium was cultured on agar medium (CGA) with different concentrations of glucose (10, 8, 6 and 4 g/dm3 ). In order to study the effect of laser irradiation on the growth of the fungus S. commune, the mycelium was irradiated with LED lasers: BRP — 3010-5, with red spectrum radiation with a wavelength of 635 nm; BBP — 3010-5 is a blue spectrum with a wavelength of 405 nm and BGP — 3010-5 is a green spectrum with a wavelength of 532 nm. Effective modes of photoactivation of mycelium by coherent light are determined. The results are obtained, which allow to state that the most effective for all studied strains of this species is irradiation of the mycelium with red light with a wavelength of 635 nm (irradiation energy 51.1 mJ/cm2 ) lasting 10 s. Under these irradiation conditions for strains S.c.-01, S.c.-02, S.c.-03 S. commune the rate of radial growth of the mycelium increased from 63.8 to 84.3%. Laser irradiation of the mycelium with blue light with a wavelength of 405 nm (irradiation energy 51.1 mJ/cm2 ) for 10 s was also effective. The average growth rate increased for strains S.c.-03, S.c.-01 and S.c.-02 by 71.8 — 52.7%. The optimal concentration of glucose (8 g/dm3 ), the value of which is less than the standard (10 g/dm3 ), which is used for CGA medium, was determined. It was found for the first time that the use of CGA medium with a glucose concentration of 8 g/dm3 in combination with laser irradiation with red (wavelength 635 nm) or blue (405 nm) light with an irradiation energy of 51.1 mJ/cm2 can significantly increase the average radial velocity mycelial growth.