Intelligent visual detection of OTC enabled by a multicolor fluorescence nanoprobe: Europium complex functionalized carbon dots

Abstract

It is of great significance to realize ultra-sensitive and visual detection of oxytetracycline (OTC) residues, especially for public health and environmental safety. In this study, a multicolor fluorescence sensing platform (CDs-Cit-Eu) for OTC detection was constructed by using rare earth europium complex functionalized carbon dots (CDs). The blue-emitting CDs (λem = 450 nm) prepared by one-step hydrothermal method using nannochloropsis were not only the scaffold of Eu3+ ion coordination, but also the recognition unit of OTC. After adding OTC to the multicolor fluorescent sensor, the emission intensity of CDs decreased slowly, and the emission intensity of Eu3+ ions (λem = 617 nm) enhanced significantly, accompanying by a significant color change of the nanoprobe from blue to red. The detection limit of the probe for OTC was calculated to be 3.5 nM, manifesting ultra-high sensitivity towards OTC detection. In addition, OTC detection in real samples (honey, lake water, tap water) was successfully achieved. Moreover, a semi-hydrophobic luminescent film SA/PVA/CDs-Cit-Eu was also prepared for OTC detection. With the help of smartphone color recognition APP, real-time intelligent detection of OTC was realized.