Integration of transcriptome and proteome analysis reveals the mechanism of freezing tolerance in winter rapeseed

Jiaping Wei,Ying Wang,Hui Li,Xiaoyun Dong,Zigang Liu,Guoqiang Zheng,Sushuang Liu

doi:10.1007/s10725-021-00763-z

Jiaping Wei, Ying Wang + Show 5 more

Open Access

https://doi.org/10.1007/s10725-021-00763-z

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Abstract

Winter rapeseed seedlings are susceptible to low temperature during overwintering in Northwest China, leading to reduced crops production. Freezing stress is one of the main environmental stresses in Northwest China from late autumn to early spring, an eventful period for overwinter survival rate of winter rapeseed. However, the molecular mechanism of freezing tolerance formation is still very backward in winter rapeseed. In this study, using a pair of freezing-sensitive and freezing-resistant cultivars NQF24 and NTS57, the exhaustive effects of freezing stress on freezing tolerance formation were evaluated by analyzing leaf at the levels of transcriptome, proteome, physiology and ultrastructure. There were 8497 and 7358 differentially expressed genes (DEGs) and 418 and 573 differentially abundant proteins (DAPs) identified in the leaf of NQF24 and NTS57 under freezing stress, respectively. Function enrichment analysis showed that most of the enriched DEGs and DAPs were associated with plant hormones signal transduction, fatty acid metabolism, ribosome, plant-pathogen interaction and secondary metabolites biosynthesis. Freezing tolerance is formed by enhanced signals transduction, increased the biosynthesis of protein and secondary metabolites, enhanced reactive oxygen species (ROS) scavenging, more osmolytes, lower lipid peroxidation, and stronger cell stability. These results can be taken as selection indicators in freezing tolerance breeding program in rapeseed.

Highlights

Freezing stress (< 0°C) has profound and diverse effects on plant growth and development, it poses a serious threat to the geographical distribution of plant species (Ding et al 2019a), it can cause ice formation in the apoplast of plant tissues, the accumulation of intracellular ice physically disrupts the cell membrane (Shi et al 2018), leading to severe cell dehydration
These results indicated that higher reactive oxygen species (ROS) scavenging activities and more osmotic regulation substances accumulation, and lower lipid peroxidation might create stronger freezing resistance in cultivar NS under freezing stress
The proteins database of Brassica napus downloaded from the NCBI, and the list of all genes and proteins identified in transcriptome and proteome, respectively; Table S2

Summary

Introduction

Freezing stress (< 0°C) has profound and diverse effects on plant growth and development, it poses a serious threat to the geographical distribution of plant species (Ding et al 2019a), it can cause ice formation in the apoplast of plant tissues, the accumulation of intracellular ice physically disrupts the cell membrane (Shi et al 2018), leading to severe cell dehydration. Its growth is persecuted to low temperature in Northwest China, which prevents winter rapeseed from overwinter and propagate, leading to severe reduction of crops yield. The studies by Xu et al (2018) and Zeng et al (2018) suggested the impacts on the roots and leaves of two winter rapeseed cultivars after freezing treatment by isobaric tags for relative and absolute quantification. Pu et al (2019) reported the effects on the freezing resistance formation in the leaves of two rapeseed cultivars after freezing treatment by high-throughput RNA sequencing (RNA-seq). All the above researches are based on traditional data-dependent acquisition (DDA) models, which might result in lower accuracy in the quantification of low abundance proteins. Our recent study obtained a freezing-responsive molecular network after short-term successive freezing treatment by the sequencing-based transcriptome and DIA-based proteome (Wei et al 2021)

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