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Abstract
The mechanism of integration of λ bio ll, which is deleted of all the known λ recombination genes, was studied using bio deleted hosts as recipients. The presence of rec BC DNase and exo I in the recipient cells affected the fate of λ bio ll DNA. In nine of ten immλ + transductants, insertion of the λ bio ll genome took place somewhere between J and N and the remaining one had abnormally permuted prophage λ. In this lysogen (#42), the sequence of prophage genes was similar to that of vegetative phage λ. The properties of lysogen #42 were compared with those of other lysogens.
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