Integration of soil solarization and chemical sterilization with beneficial microorganisms for the control of white root rot and growth of nursery apple

Abstract

White root rot ( Dematophora necatrix (Mart.)) is a serious disease of apple ( Malus domestica Borkh) in nurseries and orchards in India. In 2002 and 2003, field experiments were conducted to integrate soil solarization with native isolates of Azotobacter chrococcum and vesicular–arbuscular mycorrhizal fungi and observe its effect on the incidence of white root rot and growth of the saplings. Apple seeds coated with two native isolates of A. chrococcum (AZUHF 1 and AZUHF 2) were sown in plots inoculated with 4 native isolates of va-mycorrhiza, i.e. AMUHF 1 ( Glomus fesiculatum), AMUHF 2 ( Glomus macrocarpum), AMUHF 3 ( Glomus mosseae) and AMUHF 4 ( Gigaspora sp.) in 14 different combinations and these plots were solarized with transparent polyethylene mulch (25 μm thick) for 40 days in summer months. Soil solarization resulted around 9 °C higher temperature with average maximum temperature of 38–39 °C. Inoculation of saplings with AMUHF 1 isolate of va-mycorrhiza and AZUHF 1 isolate of A. chrococcum and then their planting in solarized soil was found most effective with no incidence of white root rot in comparison to 33.6–35.4% in control accompanied with 78–113% increase in shoot length and 81.6–84.3% increase in root length. Shoot and root length of the saplings was 9.6–10.6 and 9.2–16.0% higher, respectively, in solarized plots in comparison to sterilized plots.