Abstract
AbstractAmino acids (AAs) comprise fundamental components of the human organism since they participate in several physiological functions. Numerous methods have been developed to quantify AAs in biological fluids due to the challenges posed by matrix handling (limited sample quantities, complexity) and their physicochemical properties (protein binding, low detectability, and high polarity). This study developed and validated an LC–MS method using ion‐pairing chromatography, a sequential window acquisition of all theoretical mass spectra approach and a derivatization kit for AA analysis of physiological fluids to simultaneously quantify plasma AAs and provide data for untargeted analytical approaches. Finally, proof of concept was demonstrated through the analysis of 450 samples from a dietary intervention study with protein‐enriched wheat biscuits.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
