Integrating carbon dots and gold/silver core-shell nanoparticles to achieve sensitive detection of dopamine with fluorometric/colorimetric dual signal.

Abstract

Dopamine (DA) is a potent neuromodulator in the brain that affects a wide range of motivated behaviors. Abnormal concentration of DA is related to a variety of diseases. Hence, it is imperative to establish a rapid and precise method for quantifying DA. In this work, we integrate orange-yellow emissive carbon dots (CDs) with target-induced silver deposition on gold nanoparticles (Au NPs), forming gold/silver core-shell nanoparticles (Au@Ag NPs), to construct a fluorometric and colorimetric dual-signal sensor for sensitive detection of DA. Au NPs and silver ions (Ag+) have minimal effect on the fluorescence of CDs. DA can reduce the silver ions to Ag(0) on the surface of the Au NPs to form a silver shell, resulting in the blue-shift of theabsorbance peak from 520 to 416nm, which overlaps with the excitation spectrum of CDs. As a result, the system color turns from pink to orange-yellow, and the fluorescence of CDs is quenched due to the strong inner filter effect. The linear range of the colorimetry is 0.5-18μM with a limit of detection (LOD) of 0.41μM, while the linear range for the fluorometry method is 0.5-14μM with a LOD of 0.021μM. This method demonstrates notable advantages including a low detection limit, rapid response time, and straightforward operation in practical samples, showing great potential in biomedical analysis.