Abstract
This paper presents the development of a lab-on-chip, based on thin-film sensors, suitable for DNA treatments. In particular, the system performs on-chip DNA amplification and separation of double-strand DNA into single-strand DNA, combining a polydimethylsiloxane microfluidic network, thin-film electronic devices, and surface chemistry. Both the analytical procedures rely on the integration on the same glass substrate of thin-film metal heaters and amorphous silicon temperature sensors to achieve a uniform temperature distribution (within ±1 °C) in the heated area and a precise temperature control (within ±0.5 °C). The DNA separation also counts on the binding between biotinylated dsDNA and a layer of streptavidin immobilized into a microfluidic channel through polymer-brushes-based layer. This approach results in a fast and low reagents consumption system. The tested DNA treatments can be applied for carrying out the on-chip systematic evolution of ligands by exponential enrichment process, a chemistry technique for the selection of aptamers.
Highlights
L ABS-ON-CHIPS (LoCs) are miniaturized analytical systems, which are able to simplify complex laboratory procedures integrating several biochemical procedures on a single chip
This paper is organized as follows: Section II reports the details of the LoC structure and fabrication; Section III discusses the experimental results focusing on the DNA amplification through the polymerase chain reaction (PCR) on chip and on the double-stranded DNA (dsDNA) separation through a microfluidic chip functionalized with streptavidin; Section IV draws the conclusions
During the DNA amplification, the PCR-chip is placed on the SoG, aligning the valves and the chamber with the heaters (Fig. 3)
Summary
L ABS-ON-CHIPS (LoCs) are miniaturized analytical systems, which are able to simplify complex laboratory procedures integrating several biochemical procedures on a single chip. PCR in LoC devices has been largely reported in the literature [10]–[13], only a few examples of the miniaturized SELEX process were reported [14], [15] These systems are based on microfluidic chip in which magnetic beads functionalized and located into a microfluidic channel are brought into contact with the target molecule. Other works show the aptamer selection by coated magnetic nanoparticles, PCR amplification, and double-stranded DNA (dsDNA) denaturation in the same chamber of a single microfluidic chip [16] These devices combine microfluidic and microelectromechanical system technologies for the miniaturization and automation of the SELEX process with implications in terms of reduction of time and improved efficiency of the whole process. This paper is organized as follows: Section II reports the details of the LoC structure and fabrication; Section III discusses the experimental results focusing on the DNA amplification through the PCR on chip and on the dsDNA separation through a microfluidic chip functionalized with streptavidin; Section IV draws the conclusions
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: IEEE Transactions on Components, Packaging and Manufacturing Technology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
