Integrated Learning: Screening Optimal Biomarkers for Identifying Preeclampsia in Placental mRNA Samples.

Rong Guo,Yiding Wang,Heze Xu,Dan Liu,Xin Zhou,Zhixia Teng,Waqas Haider Bangyal

doi:10.1155/2021/6691096

Rong Guo, Yiding Wang + Show 5 more

Open Access

https://doi.org/10.1155/2021/6691096

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Abstract

Preeclampsia (PE) is a maternal disease that causes maternal and child death. Treatment and preventive measures are not sound enough. The problem of PE screening has attracted much attention. The purpose of this study is to screen placental mRNA to obtain the best PE biomarkers for identifying patients with PE. We use Limma in the R language to screen out the 48 differentially expressed genes with the largest differences and used correlation-based feature selection algorithms to reduce the dimensionality and avoid attribute redundancy arising from too many mRNA samples participating in the classification. After reducing the mRNA attributes, the mRNA samples are sorted from large to small according to information gain. In this study, a classifier model is designed to identify whether samples had PE through mRNA in the placenta. To improve the accuracy of classification and avoid overfitting, three classifiers, including C4.5, AdaBoost, and multilayer perceptron, are used. We use the majority voting strategy integrated with the differentially expressed genes and the genes filtered by the best subset method as comparison methods to train the classifier. The results show that the classification accuracy rate has increased from 79% to 82.2%, and the number of mRNA features has decreased from 48 to 13. This study provides clues for the main PE biomarkers of mRNA in the placenta and provides ideas for the treatment and screening of PE.

Highlights

Preeclampsia (PE) is a pregnancy-specific syndrome that affects 3-5% of pregnant women and is characterized by edema, hypertension, and proteinuria [1]
We introduced the area under the curve (AUC) as an indicator to measure the effectiveness of the model
The optimal subset consisting of 13 mRNA attributes was obtained (HTRA4, PROCR, MYCN, ERO1A, EAF1, PPP1R16B, CRH, FLNB, PIK3CB, PLAAT3, FBN2, RFLNB, and TKT)

Summary

Introduction

Preeclampsia (PE) is a pregnancy-specific syndrome that affects 3-5% of pregnant women and is characterized by edema, hypertension, and proteinuria [1]. PE is a multifactor and multigene disease with a family genetic predisposition: assuming a mother had PE, the incidence of PE in her daughters is 20-40%. PE makes women more susceptible to cardiovascular disease later in life and may affect brain function. It remains a major factor in maternal and newborn morbidity and mortality [2]. The placenta is an important organ shared by the mother and the fetus. It has important biological functions such as substance exchange, metabolism, and barrier function. Abnormal placental function can lead to pregnancy diseases such as PE. Many physiological and biochemical processes related to placental function are coordinated by proteins that form complex networks in the placenta, and the production of proteins requires the participation of RNA

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