Integrated effects of operational temperature, HRT, and influent ammonium concentration on a CANON coupling with denitrification process treating for digested piggery wastewater: performance and microbial community.

Abstract

In this study, an improved system called the completely autotrophic nitrogen removal over nitrite (CANON) process was presented and coupled with denitrification for the treatment of digested piggery wastewater (DPW). The effects of operating parameters, including hydraulic retention time (HRT) (1.6d → 1.0d), influent NH4+-N concentration (350mgL-1 → 600mgL-1), and temperature (41℃ → 17℃), on the nitrogen removal performance and response characteristics of microbial population were investigated. Results showed that all considered parameters caused a remarkable effect on NH4+-N and total nitrogen removal efficiencies, and the chemical oxygen demand was more markedly affected by temperature. Candidatus_Kuenenia, Candidatus_Brocadia, Denitratisoma, norank_o_Xanthmonadales, norank_p_WWE3, and SM1A02 were the dominant genera influencing nitrogen removal in the improved CANON system for treating DPW. Redundancy discriminant analysis showed that the biological structure was positively correlated with the influent ammonium concentration, temperature, and HRT. The relative abundance of Candidatus_Kuenenia was perfectly correlated with HRT and temperature. However, environmental factors did not affect Candidatus_Brocadia and norank_p_WWE3. norank_c_Ardenticatenia, SM1A02, and norank_f_SJA-28 were all positively correlated with influent ammonium nitrogen concentration, but not correlated with HRT and temperature. The improved CANON process realized the nitrogen removal under high ammonium (NH4+-N) concentration and low C/N wastewater.