Abstract
Introduction:: Bermudagrass (Cynodon dactylon L.) simultaneously has three types of stems: shoots, stolons, and rhizomes, which lays the basis for the fast clonal growth of this important warm-season turfgrass species. However, the mechanisms underlying the differentiation, growth, and development of the three types of stems remain unclear. Methods:: In this study, the annotation information of the assembled bermudagrass genome was used to reanalyze the mass spectrometry raw data generated in the comparative proteomics analysis of bermudagrass shoots and stolons as well as stolons and rhizomes. One-way analysis of variance and the Student-Newman-Keuls test was performed to identify the Differentially Expressed Proteins (DEPs) in paired comparison of shoots versus stolons, shoots versus rhizomes, and stolons versus rhizomes. Results:: A total of 3190 proteins were simultaneously expressed in the three types of stems, whereas 135, 1012, and 876 DEPs were identified between shoots and stolons, shoots and rhizomes, and stolons and rhizomes, respectively. Venn diagram analysis indicated that 23 DEPs were simultaneously identified in the three paired comparisons. Functional enrichment analysis indicated endocytosis and terpenoid backbone biosynthesis to be the most significantly DEP-enriched biochemical pathways among the three types of stems. Conclusion:: The results of this study not only provided new insights into the specialization of shoots, stolons, and rhizomes in bermudagrass, but also pointed out the importance of high-quality genome assembly and annotation in proteomics research.
Published Version
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