Abstract
Little has been known about the role of long non-coding RNA (lncRNA) involves in change of aged meniscus. Microarray analyses were performed to identify lncRNAs and mRNAs expression profiles of meniscus in young and aging adults and apple bioinformatics methods to analyse their potential roles. The differentially expressed (DE) lncRNAs and mRNAs were confirmed by qRT-PCR. A total of 1608 DE lncRNAs and 1809 DE mRNAs were identified. Functional and pathway enrichment analyses of all DE mRNAs showed that DE mRNAs were mainly involved in the TGF-beta, Wnt, Hippo, PI3K-Akt signaling pathway. The expressions of TNFRSF11B and BMP2 were significantly upregulated in aging group. LASSO logistic regression analysis of the DE lncRNAs revealed four lncRNAs (AC124312.5, HCG11, POC1B-AS1, and AP001011.1) that were associated with meniscus degradation. CNC analysis demonstrated that AP001011 inhibited the expression of TNFRSF11B and AC1243125 upregulated the expression of TNFRSF11B. CeRNA analysis suggested that POC1B-AS1 regulates the expression of BMP2 by sponging miR 130a-3p, miR136-5p, miR 18a-3p, and miR 608. Furthermore, subcellular localization and m6A modification sites prediction analysis of these four lncRNAs was performed. These data lay a foundation for extensive studies on the role of lncRNAs in change of aged meniscus.
Highlights
As a crucial part of the tibiofemoral articulation and an articular cartilage of the knee joint, the meniscus protects the underlying articular cartilage, contributing to normal function of the knee joint and protecting the articular surfaces (Noble and Turner, 1986; Huang et al, 2019)
In order to understand the influence of age factors on meniscus tissues, we analyzed the changes of long non-coding RNA (lncRNA) and mRNA expression profiles in meniscus tissues of young and aging people by microarray analysis
After verification by qRT-RCR, we found the expression of metalloproteinase-2 (MMP-2) and type I α 2 the expression of colagen (COL1A2) were significantly decreased in the aging group
Summary
As a crucial part of the tibiofemoral articulation and an articular cartilage of the knee joint, the meniscus protects the underlying articular cartilage, contributing to normal function of the knee joint and protecting the articular surfaces (Noble and Turner, 1986; Huang et al, 2019). Many changes occur in articular cartilages with aging, including dysregulated expression of related genes, deterioration of mechanical properties and degenerative changes in the morphology (Sacitharan and Vincent, 2016). Knee cartilage thickness is negatively correlated with age All these changes aggravate the course of osteoarthritis, increase the incidence of meniscal tears in the elderly population, and seriously affect people’s quality of life. Studies have shown that lncRNAs play important roles in the aging process of tissues and organs. In order to investigate the molecular mechanisms of age-related change of meniscus and further explore potential biologic therapeutic targets, we performed microarray analysis of meniscus to determine lncRNAs and mRNAs expression profiles in young and aging adults. We carried out bioinformatics analysis of differentially expressed molecules to identify key molecules and functional pathways in the aged meniscus tissues
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