Abstract
Intact vs. homogenized subsampling: testing impacts of pre-extraction processing of multi-species samples on invasive species detection
Highlights
High throughput sequencing (HTS), metagenetics or metabarcoding, is a popular tool for assessing aquatic biodiversity (Valentini et al 2016; Borrell et al 2017; Ransome et al 2017)
When examining the percent OTU richness captured per subsample, we found that a single subsample was only capable of capturing 43.5–56% of the richness (Figure 2)
When examining the percent OTU richness per subsample, we found that a single subsample was only capable of capturing 23.8–46.7% of the richness of low abundance taxa (Figure S3)
Summary
High throughput sequencing (HTS), metagenetics or metabarcoding, is a popular tool for assessing aquatic biodiversity (Valentini et al 2016; Borrell et al 2017; Ransome et al 2017). This analysis shows that homogenized subsamples had a greater number of low abundance OTUs (Figure 6E, F), though this is likely impacted by the varying richness in the initial sample (i.e., samples 656 and 755 appear to have higher richness than the other samples chosen for intact subsampling).
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