Abstract
Pancreatic β-cells have an intrinsic oscillatory Ca 2+ activity supposed to be synchronized among the islets by cytoplasmic Ca 2+ transients elicited by nonadrenergic, noncholinergic (NANC) neurons. To improve the understanding of this process, the cytoplasmic Ca 2+ concentration ([Ca 2+] i) was measured in two insulin-releasing cell lines using dual wavelength microfluorometry and the indicator fura-2. INS-1 cells but not RINm5F cells were found to generate transients of [Ca 2+] i in the presence of the Ca 2+ channel blocker methoxyverapamil. These transients differed from those occurring in native β-cells persisting in the presence of thapsigargin or during prolonged exposure to ATP. Moreover, the [Ca 2+] i transients were poorly synchronized whether or not the INS-1 cells had physical contact. If appearing in native β-cells, the type of [Ca 2+] i transients now observed may interfere with the coordination of the β-cell rhythmicity evoked by NANC neurons.
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More From: Biochemical and Biophysical Research Communications
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