Insilico analysis of Myostatin protein of Labeo calbasu

Abstract

Myostatin is a protein released in autocrine fashion which exerts negative effect for skeletal muscle growth during myoblast proliferation and differentiation in teleost fishes. Myostatin is a member of the TGF-β family encoded by MSTN gene, also known as growth differentiation factor 8(GDF-8). MSTN gene knock-out exhibited hypertrophy and hyperplasia of skeletal muscle, but over expression or systemic administration of myostatin causes muscle atrophy. Blocking of myostatin activity helps in generating higher muscle growth rate and mutation in MSTN gene creates double-muscle phenotype. Therefore, insilico analysis of Myostatin in an Indian major carp species Labeo calbasu has carried out in the present study for understanding structural organization of the concerned protein. Amino acid sequence heaving the accession number AEN75195.1 was retrieved from NCBI. The sequence was subjected to Expasy ProtParam, PSIPRED and GORIV, PDB sum and SWISSMODEL/ Workspace for finding out the physicochemical properties, secondary structure prediction and template selection procedure respectively. The predicted 3D structure of Myostatin by Swiss model server was validated through RAMPAGE server and then submitted to Protein Model Database (PMDB) heaving the ID No: PM0081037. The extracellular location was also analysed by Hum-mPLoc 2.0. The structural examination of Myostatin will help in development of antagonistic protein or inhibitor, which can shed a new direction for the growth promotion of piscine species during various periods of life cycle.