In-silico analysis of a halophilic bacterial isolate-Bacillus pseudomycoides SAS-B1 and its polyhydroxybutyrate production through fed-batch approach under differential salt conditions

Abstract

Polyhydroxybutyrate (PHB) is a natural biopolymer and a viable substitute for petroleum-derived polymers that possess immense potential for diverse applications. In the present study, PHB was produced by a halophilic bacteria identified as Bacillus pseudomycoides SAS-B1 by 16S rRNA gene sequencing. The bacterial genome was evaluated through complete genome sequencing, which elucidated a 5,338,308 bp genome with 34.88 % of G + C content and 5660 genes. Other genome attributes were analyzed such as functional profiling, gene ontology, and metabolic pathways. Genes involved in PHB biochemical pathway were identified such as phaA, phaB, and phaC. Furthermore, sodium-dependent transporters and other ATP-binding genes were identified in the genome that may be involved in sodium uptake during saline conditions. The PHB production by B. pseudomycoides SAS-B1 was examined under differential salt conditions. The PHB yield was increased from 3.14 ± 0.02 g/L to 6.12 ± 0.04 g/L when salinity was increased upto 20 g/L with intermittent feeding of glucose and corn steep liquor. FTIR, NMR, and GC–MS studies elucidated the presence of desired functional groups, molecular structure, and monomeric compositions of PHB respectively. Further, TGA revealed the thermal stability of the recovered PHB upto (220−230) °C and has a crystallinity index of upto 33 ± 0.5 % as confirmed by XRD analysis.