Abstract
Abstract As a new type of ionic fluids that are inexpensive and environmentally friendly, deep eutectic solvents (DESs) have recently attracted a broad interest in many fields including biocatalysis. In this study, 24 DESs were prepared by mixing two cholinium salts (cholinium chloride (ChCl) and cholinium acetate (ChAc)) with four H-bond donors (HBDs) (urea, glycerol, acetamide, ethylene glycol) at three molar ratios, and their effects on the activity, stability and structure of horseradish peroxidase (HRP) have been investigated. The ChCl-based DESs have been found to be superior to the ChAc-based ones in terms of promoting the HRP activity. For the DESs composed of the same salt and same HBD, an increase in the HRP activity has been observed with a higher salt/HBD molar ratio of 1:2
Published Version
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