Abstract
Endophytic actinobacteria play an important role in growth promotion and development of host plant by producing enormous quantities of novel bioactive natural products. In the present investigation, 169 endophytic actinobacteria were isolated from endospheric tissues of Rhynchotoechum ellipticum. Based on their antimicrobial potential, 81 strains were identified by 16rRNA gene analysis, which were taxonomically grouped into 15 genera. All identified strains were screened for their plant growth promoting attributes and, for the presence of modular polyketide synthases (PKSI, PKSII and nonribosomal peptide synthetase (NRPS) gene clusters to correlate the biosynthetic genes with their functional properties. Expression studies and antioxidant potential for four representative strains were evaluated using qRT-PCR and DPPH assay respectively. Additionally, six antibiotics (erythromycin, ketoconazole, fluconazole, chloramphenicol, rifampicin and miconazole) and nine phenolic compounds (catechin, kaempferol, chebulagic acid, chlorogenic acid, Asiatic acid, ferulic acid, arjunic acid, gallic acid and boswellic acid) were detected and quantified using UHPLC-QqQLIT-MS/MS. Furthermore, three strains (BPSAC77, 121 and 101) showed the presence of the anticancerous compound paclitaxel which was reported for the first time from endophytic actinobacteria. This study provides a holistic picture, that endophytic actinobacteria are rich bacterial resource for bioactive natural products, which has a great prospective in agriculture and pharmaceutical industries.
Highlights
Endophytic actinobacteria play an important role in growth promotion and development of host plant by producing enormous quantities of novel bioactive natural products
One hundred and sixty-nine endophytic actinobacterial isolates were obtained from root, stem and leaf parts of R. ellipticum based on colony morphology and formation of color by aerial and substrate mycelia
Endophytic microorganisms like endophytic actinobacteria inhabit within the plant tissues acquired certain unique characteristics that enable them to flourish without any obvious infectious symptoms to the host
Summary
Endophytic actinobacteria play an important role in growth promotion and development of host plant by producing enormous quantities of novel bioactive natural products. 169 endophytic actinobacteria were isolated from endospheric tissues of Rhynchotoechum ellipticum Based on their antimicrobial potential, 81 strains were identified by 16rRNA gene analysis, which were taxonomically grouped into 15 genera. Endophytic microbial communities associated within medicinal plants have been proven to have great potential as producers of novel bioactive compounds, which have a great prospective in agriculture, pharmaceutical and other industries[3,4,15]. Besides antimicrobial and antiviral potential, the endophytic actinobacteria obtained from medicinal plants showed a significant effect as larvicidal[23], antimalarial[24,25], antitumor[26], antidiabetic[27] and as plant growth promoters[28]
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