Abstract
(1) Background: Phytohabitans is a recently established genus belonging to rare actinomycetes. It has been unclear if its members have the capacity to synthesize diverse secondary metabolites. Polyketide and nonribosomal peptide compounds are major secondary metabolites in actinomycetes and expected as a potential source for novel pharmaceuticals. (2) Methods: Whole genomes of Phytohabitans flavus NBRC 107702T, Phytohabitans rumicis NBRC 108638T, Phytohabitans houttuyneae NBRC 108639T, and Phytohabitans suffuscus NBRC 105367T were sequenced by PacBio. Polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) gene clusters were bioinformatically analyzed in the genome sequences. (3) Results: These four strains harbored 10, 14, 18 and 14 PKS and NRPS gene clusters, respectively. Most of the gene clusters were annotated to synthesis unknown chemistries. (4) Conclusions: Members of the genus Phytohabitans are a possible source for novel and diverse polyketides and nonribosomal peptides.
Highlights
The discovery of new bioactive secondary metabolites remains one of the most important tasks for current pharmaceutical developments
Prediction of products based on secondary metabolite-biosynthetic gene clusters (smBGCs), such as Polyketide synthase (PKS)
We sequenced whole genomes in four type strains of the genus Phytohabitans, which have not been studied by genome sequence-based strategies, by PacBio, analyzed their PKS and nonribosomal peptide synthetase (NRPS)
Summary
The discovery of new bioactive secondary metabolites remains one of the most important tasks for current pharmaceutical developments. Post-genomic studies for actinomycetes revealed that each actinomycete harbors in general diverse secondary metabolite-biosynthetic gene clusters (smBGCs) even if the strain had been reported to produce only few compounds [4]. This led to an approach based on analyzing smBGCs in whole genomes, called genome-mining, to search new natural products, resulting in effective isolation of new secondary metabolites [5]. To elucidate the capacity to synthesize polyketide and nonribosomal peptide compounds, we conducted whole genome-sequencing for type strains of these species and bioinformatically analyzed PKS and NRPS gene clusters
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