Insight into the roles of the ER-associated degradation E3 ubiquitin ligase HRD1 in plant cuticular lipid biosynthesis

Abstract

Cuticular lipids consisting of cutin and wax coat aerial plant surfaces providing protection against biotic and abiotic stresses. Although much progress has been made on comprehending the regulation of plant cuticular lipid biosynthesis, their functional relevance in plant protection merits further investigation of potential regulators of their synthesis. HRD1 and DOA10 mediate two major Endoplasmic Reticulum-Associated Degradation (ERAD) pathways in yeast and also regulate common pathways during lipid metabolism. However, their roles in plant lipid metabolism are not well studied. CER9, an Arabidopsis homolog of DOA10, is known to play important roles in cuticular lipid biosynthesis. This prompted us to determine if HRD1 also plays a role in regulation cuticular lipid biosynthesis. Here we report that an Arabidopsis hrd1a hrd1b double mutant is impacted in the accumulation of both cutin and cuticular waxes including a large increase in total stem cutin with a concomitant decrease in stem wax content. We further investigated genetic relationship between HRD1A/1B- and CER9-mediated ERAD pathways with regard to cuticular lipid synthesis. Surprisingly, simultaneous mutation of HRD1 and CER9 revealed additive effects on stem wax synthesis, but not stem cutin synthesis. Collectively, our study advances our understanding of the ERAD regulatory roles in cuticular lipid synthesis identifying HRD1 as an important player in the regulated deposition of Arabidopsis stem cuticular lipids.