Abstract
Next-generation technologies have prompted efforts towards generating a large repertoire of whole-genome sequences. The dermatophyte Arthroderma vanbreuseghemii has been considered as a good model in which to conduct molecular biological studies on this fungal group. Despite the considerable repertoire of molecular tools developed for this fungus, the lack of genomic data has represented a major limitation, preventing effective implementation of those tools. Herein, the authors report the first draft whole-genome sequence of this dermatophytic species. The size of the draft genome was 23 Mb, exhibiting a GC content of 48.1%. Given the significance of secreted proteases in tissue invasion, a comparative analysis of genes encoding extracellular proteases was performed between A. vanbreuseghemii and other dermatophytes. Furthermore, genes that might be involved in DNA repair also were compared among dermatophytes. Moreover, the complete mitochondrial genome of A. vanbreuseghemii was obtained and shown to consist of 24,287 bp with a GC content of 24%. In conclusion, the availability of genomic data for A. vanbreuseghemii is expected to facilitate the implementation of the molecular tools established for this fungus, enhancing our understanding of the biology of dermatophytes.
Highlights
Dermatophytes are fungal agents that invade the outer layers of the skin
The scarcity of genomic data on this strain has precluded the effective exploitation of these molecular tools
We demonstrated that the proteolytic activity of TIMM 2789 in skimmed milk is primarily the result of the activity of metalloprotease-4 (MEP4)[15]
Summary
Dermatophytes are fungal agents that invade the outer layers of the skin. Infections by this group are wide-spread around the world and are known to worsen the quality of human and animal lives while imposing a large economic burden for therapy[1,2,3]. Strain TIMM 2789 can be used as a model organism for the study of dermatophytes This strain has been widely employed in genetic research, in studies aimed at developing essential molecular tools. These tools include transformation methods, selectable markers, and a marker-recycling system, as well as the generation of TIMM 2789. Derivatives with improved homologous recombination (HR) efficiencies as a result of disruption of the strain’s non-homologous end-joining (NHEJ) pathway[10,11,12,13,14,15] Application of these molecular tools has long been hampered by a lack of genomic data. The authors conducted a comparative study based on exploring several gene families and pathways that are of biological or medical importance
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