Abstract
Plasmids that contain a d(pCpG) 13 · d(pCpG) 13 insert instead of a lac operator show a 34-fold decrease of β-galactosidase synthesis. The same sequence causes a 24-fold decrease when inserted between codons 5 and 6 of the lacZ gene. In such constructs with d(pCpG) n · d(pCpG) n inserts, β-galactosidase activity decreases approximately 1·6-fold per d(pCpG) · d(pCpG) unit when n ranges from 5 to 16.
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