Abstract
Abstract The Steindachneridion parahybae is an endangered catfish from Brazil and strategies applied for gametes optimization are necessary. The aim of this study was to assess inseminating doses and water volume upon the fertilization, hatching rates and percentage of normal larvae in S. parahybae . Was used a randomized design in factorial scheme (4×4) with four inseminating doses: 1.0×104, 1.0×105, 1.0×106, 1.0×107spermatozoa oocyte-1 and four volumes of water: 1, 35, 65 and 95mL of water g-1 of oocytes. The combination of doses and volumes were performed in triplicates (n=48). Each incubator (1.5L of useful volume) with 1g of oocytes was considered as an experimental unit. Significant interaction between inseminating doses and volumes of water to the values of the fertilization rates and quadratic effect of doses and volume for the values of hatching rates were observed. The doses and volumes did not influence the percentage of normal larvae (87.70±5.06%). It is recommended the use of 5.5×106 spermatozoa oocyte-1 and 1mL of water g-1 of oocytes during in vitro fertilization procedure. These results allowed us to develop new biotechnological strategies applied to the conservation of S. parahybae .
Highlights
Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae), the surubim-do-Paraíba, is a endemic catfish to the Paraíba do Sul River basin (Garavello, 2005)
The highest values could be verified in the application of the inseminating doses of 5.4×106 spermatozoa oocyte-1 in 1.0mL of water g-1 of oocytes, up to 6.4×106 spermatozoa oocyte-1 in 95.0mL of water g-1 of oocytes (Fig. 1)
The sperm production found in this study was higher than the one verified for the same species by Caneppele et al (2015) and the computerized sperm characteristics were higher than the ones verified by Sanches et al (2013) for fresh semen, and by Araújo (2011) for cryopreserved semen
Summary
Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae), the surubim-do-Paraíba, is a endemic catfish to the Paraíba do Sul River basin (Garavello, 2005). The authors verified the ovulation in a period of 250 degree-hours with 85% of fertilized oocytes. More studies about gamete characterization and reproductive management during the process of artificial fertilization, as well as about the reproductive biotechniques in captivity are necessary for the preservation of this species. They may contribute directly to the rational use of gametes, increasing the success of the artificial propagation, like the application of effective inseminating doses (Rurangwa et al, 2004)
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