Abstract
An in vitro study was designed to determine the extent of Salmonella enterica serovar enteritidis survival and growth permissiveness in egg components isolated from shell eggs held at refrigeration temperature over an 8 week time period. Eggs were collected from a commercial laying facility at one-week intervals for eight weeks and stored at refrigeration temperature. After storage, eggs were dipped in ethanol, cracked aseptically and separated into yolk and albumen samples. S. enteritidis resistant to novobiocin and nalidixic acid were inoculated on to the surface of the yolk membrane at a concentration of approximately 10 6 CFU mL¯ 1 . Yolks were then covered with albumen and incubated for 24 hrs at 25oC. After incubation, eggs were separated into component parts. Samples were removed from yolk, albumen and yolk membrane and diluted 10-fold in sterile phosphate buffered saline. In albumen, S. enteritidis counts were increased in weeks 3 and 8 compared to week 1 (trial 2). The frequency of eggs exhibiting net growth of S. enteritidis in albumen occurred at week 7 versus weeks 0 and 1 in trial 1 and weeks 3 and 8 versus weeks 0 and 2 in trial 2. In the membrane fraction, the frequency of eggs exhibiting net growth of S. enteritidis occurred at weeks 5 and 8 versus week 0 in trial 2. In the yolk fractions, S. enteritidis counts recovered from week 6 eggs were significantly higher (P<0.05) than those of weeks 0, 2, 3 and 7 (trial 2) and the number of detectable S. enteritidis positive eggs were greater in week 8 than week 5 in trial 1. This suggests that egg components recovered from aged eggs stored at refrigeration temperatures infrequently supported S. enteritidis net growth but generally did not inhibit survivability.
Highlights
Salmonella enterica serovar Enteritidis (S. enteritidis) accounts for over 65% of all human serotype isolates worldwide[1]
The objective of this in vitro study was to use similar vitelline membrane inoculation approaches as our previous work with S. typhimurium[24] and determine whether sufficient deterioration occurs in refrigerated eggs over an 8 week time period such that individual egg components will permit survival and net growth of S. enteritidis after inoculation on the vitelline membrane
Net increases of S. enteritidis rarely occurred in either vitelline membrane or albumen isolated from eggs stored over an 8 week period
Summary
Salmonella enterica serovar Enteritidis (S. enteritidis) accounts for over 65% of all human serotype isolates worldwide[1]. We observed that serotype S. typhimurium could survive and exhibit net growth on egg components during an 8 week storage period under refrigeration conditions[24]. We hypothesize that an increase in the refrigeration time could increase the susceptibility to S. enteritidis contamination The objective of this in vitro study was to use similar vitelline membrane inoculation approaches as our previous work with S. typhimurium[24] and determine whether sufficient deterioration occurs in refrigerated eggs over an 8 week time period such that individual egg components will permit survival and net growth of S. enteritidis after inoculation on the vitelline membrane
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