Innovative pH-dependent approach for electrochemical determination of a triple eradication therapy targeting H. Pylori infection in pharmaceutical formulation and human plasma sample: Modified electrode with Prussian blue analogue decorated multi-walled carbon nanotubes (PbA@MWCNT)

Abstract

In the present study, a sensitive and cost-effective voltammetric method was developed, optimized and applied for assessment of a new combination of a triple therapy treatment that contain tinidazole (TZ), clarithromycin (CLR) and lansoprazole (LAN) depending on different pH effect. The study was developed using electrode of carbon paste improved by Prussian blue analogue nanoparticles in addition to multi-walled carbon nanotubes (PbA-NPs/MWCNT/CPE). The characterization and morphology of the nanoparticles composite was studied for the adopted electrode by X-ray diffraction (XRD) patterns, scanning electron microscope (SEM) and Transmission Electron Microscope (TEM). Effect of different electrodes and content of PbA-NPs on the voltammetric performance of the three drugs were assessed. Differential pulse voltammetry (DPV) was estimated and linear correlations were then obtained in the range of 0.01–0.9, 1.0–10.0 and 0.5–12.0 µg/mL for LAN, CLR and TZ, respectively. The optimized method has been validated as per the ICH guidelines and found to be appropriate for the routine quality control of the studied drugs in their pharmaceutical formulation in addition to spiked human plasma. Consequently, the proposed method definitely provides an efficient, beneficial, reliable and cost-effective tool for estimation of the cited active pharmaceutical ingredients.