Abstract
The results over the test of new domestic vaccine against to the blackleg – “ЕМКАРВАК” was brought. The local productive strain C. chauvoei of Л– 7 has high immunogenicity, protective capacity and create immunity duration over 9 months. The corresponding changes in regulation technological process was enable to use of the newest data in relation to immunogenicity capacity of C. chauvoei. In particular, duration of accumulation of microbial mass of C. chauvoei in a reactor diminished from 36–42 hours to 16–18 hour, as an adsorbent is used aerosils of brand of А-300 (a production is Germany) from the calculation of 3 ± 0.1mg/sm3 culture; the supernatants was recovered by concentration of liquid after inactivation. The microbial mass of vaccine is 3.5 ± 0.3×1093. The tests of vaccine of “ЕМКАРВАК” showed that preparation was sterile, inactivated, harmless (a bioassay is on guinea-pigs), owns high antigen (the level of antibodies for the instilled animals presented 1:828 ± 257 on a 21th day after inoculation) and immunogenicity activity. The field tests of vaccine of “ЕМКАРВАК” showed that a vaccine owns high antigenicity, providing even in 12 months after her inoculation high level of specific antibodies (the level for the instilled animals presented 1:32 ± 6.0 against 1:11 ± 3.6 for control animals). The productive tests of the productive-experienced series of vaccine of “ЕМКАРВАК’ conducted in the conditions of 22 unhappy economies of the Volyn’ area on young cattle. Rejections of general (oppression, increase of temperature, loss of appetite and others like that) or changes of local character (slight swelling in place inoculation, increase of regional lymphatic knots) it was not educed. Thus, the vaccine of “ЕМКАРВАК” in the conditions of the field test appeared harmless and immunogenicity, able to support tense immunity at the receptive contingent of cattle during 9 months (term of supervision).
Highlights
Experimental Station of Epizootology of the Institute of Veterinary Medicine of the National Academy of Sciences of Ukraine, Knyaz Volodymyr Str., 18, Rivne, 33028, Ukraine
The corresponding changes in regulation technological process was enable to use of the newest data in relation to immunogenicity capacity of C. chauvoei
Duration of accumulation of microbial mass of C. chauvoei in a reactor diminished from 36–42 hours to 16– 18 hour, as an adsorbent is used aerosils of brand of А-300 from the calculation of 3 ± 0.1mg/sm3 culture; the supernatants was recovered by concentration of liquid after inactivation
Summary
The tests of vaccine of “ЕМКАРВАК” showed that preparation was sterile, inactivated, harmless (a bioassay is on guinea-pigs), owns high antigen (the level of antibodies for the instilled animals presented 1:828 ± 257 on a 21th day after inoculation) and immunogenicity activity. У статті наведено результати випробувань нової вітчизняної вакцини проти емфізематозного карбункулу “ЕМКАРВАК” із вітчизняного виробничого штаму C. chauvoei Л-7, яка створює імунітет тривалістю понад 9 місяців. Польовими випробуваннями вакцини “ЕМКАРВАК” встановлено, що вакцина володіє високою антигенністю, забезпечуючи навіть через 12 місяців після її уведення високий рівень специфічних антитіл (рівень аглютинінів у щеплених тварин становив 1:32 ± 6,0 проти 1:11 ± 3,6 у контрольних тварин). Вакцина “ЕМКАРВАК” в умовах польового випробування виявилася нешкідливою та імуногенною, здатною підтримувати напружений імунітет у сприйнятливого контингенту великої рогатої худоби протягом 9 місяців (термін спостереження).
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