Initiation of secretory activity of rat prostatic epithelium in organ culture.

Abstract

Functional differentiation of prostatic epithelium is manifested by the production of tissue specific secretory proteins. In vivo production of these proteins is dependent on the presence of serum androgens. A serum-free organ culture system was used to examine the initiation of prostatic epithelial cytodifferentiation in vitro using two rat prostate specific secretory proteins (DP-1 and probasin) as markers of epithelial cytodifferentiation. The dorsal-lateral and anterior prostatic (AP) lobes from 12-day-old rats were cultured for 6 days in serum-free medium in the presence or absence of androgens. At the start of culture, secretory proteins DP-1 and probasin were undetectable using Western blot analysis. DP-1 and probasin were produced by explants cultured in the presence of androgens but were not detected in the absence of androgens. Dose-response studies were carried out for testosterone (T), 5 alpha-dihydrotestosterone (DHT), 5 alpha-Androstan-3 alpha, 17 beta-diol (3 alpha-Adiol), and two synthetic androgens: 17 alpha-methyl-19-nortestosterone (MENT) and methyltrienolone (R1881). All androgens used were capable of inducing expression of DP-1 and probasin in vitro. T, R1881, and Ment were effective at doses of 10(-7) M to 10(-9) M, whereas both DHT and 3 alpha-Adiol were able to induce DP-1 and probasin at concentrations as low as 10(-10) M. Estrogen (17 beta-Estradiol), hydrocortisone (11 beta, 17 alpha, 21-trihydroxypregn-4-ene-3, 20-dione), and progesterone (4-pregnen-3, 20-Dione) were ineffective in inducing prostatic secretory activity. Hydroxyflutamide (alpha-alpha-alpha-trifluro-2-methyl-4'-nitro-m-lactoluidide ) blocked the induction of secretory activity elicited by T. From histological sections, it was observed that explants cultured with T exhibited tall columnar epithelial morphology with organized stromal components. Tissue sections of explants cultured without T exhibited a cuboidal to low columnar morphology with less organized stromal components when compared with glands cultured with T. A DNA synthetic index was established to measure proliferation in the explants at the end of the culture period. Explants cultured in the presence of T exhibited greater DNA synthetic activity than explants cultured in the absence of T (P < 0.05). Using this serum-free model, we can explore the mechanism for the initiation of secretory cytodifferentiation.