Initial vascularisation in the pig placenta: I. Demonstration of nonglandular areas by histology and corrosion casts.

Abstract

The vascular interrelationship of the well-established porcine placenta has previously been described from vascular casts and histology, but not its developmental stages. This study was performed using the same methods on 17 sows of well-known stages of gestation ranging from 9 1/2 to 43 days post coitum (p.c.). At the precontact stage, days 9 1/2 to 12 1/2 p.c., the subepithelial capillaries formed a wide open meshwork of variable diameter, 3-14 microns, without any difference between meso- and antimesometrial side. At the early contact and adhesion stages (days 13 to 18 p.c.), the first increase in vasculature was seen at the mesometrial side close to the embryonic disc of the very long blastocyst at day 15 p.c., 2 days after the first contact between trophoblast and maternal epithelium was seen. At day 18 p.c., the areas with dense capillaries increased markedly at the mesometrial side with the same parallel organization as seen at day 15 p.c., whereas the antimesometrial side still had a relative loose appearance comparable to the previous stages. At the early placental stages (days 20 1/2 to 23 p.c.), the capillary bed formed smooth folds, which in some areas at day 20 1/2 days developed into smaller folds or prerugae. Here the capillaries changed to convoluted forms with slightly bulbous dilations measuring about 30-35 microns in diameter. This developmental progress became more elaborate at day 23: capillaries of the low ridges of prerugae formed irregular dilations up to 50 microns in some areas. At this stage the parallel arrangement of the capillary meshwork characteristic of the previous stage was not longer discernable. By days 32-43 p.c., an increase in microscopic folding was present, and the maternal arterioles could be traced to the top of the ridges, creating the characteristic vascular architecture needed for an efficient exchange of oxygen, carbon dioxide, and nutrients of the basically developed porcine placenta.