Inhibitory effects of Sodium 1-(12-Hydroxy)octadecanyl Sulfate,an MMP-2 inhibitor,on the development of defocus-induced myopia in guinea pigs

Abstract

Objective To investigate the inhibitory effects of Sodium 1-(12-Hydroxy)octadecanyl Sulfate,an MMP-2 inhibitor,on the development of defocus-induced myopia in guinea pigs and its mechanism.Methods Thirty newborn guinea pigs were randomly divided into 3 groups,group A: -10D lens applied on right eye;group B: -10D lens and MMP-2 inhibitor treatment in right eye(9.0μg/ml,subconjunctival injection,every 3 days interval);group C: -10D lens and vehicle treatment in right eye(same volume and frequency with those of group B);there was no treatment in all the left eyes in this study.After 28 days of defocus,the refractive status was recorded and the posterior scleral sample was collected for MMP-2 zymography and western-blot analysis.Results The treated eyes of group A,B and C gained(5.73±0.36)D,(2.58±0.33)D and(6.31±0.73)D respectively,the amount of gained myopic refraction in eyes of group A was significantly less than that of eyes in group B and group C(P 0.05);the MMP-2 protein level in posterior sclera of three groups also didn't show any significant difference by western-blot analysis(p>0.05).Conclusions 1-(12-Hydroxy)octadecanyl Sulfate(9.0μg/ml,subconjunctival injection,every 3 days interval)inhibited the development of defocus-induced myopia in newborn guinea pigs,may through,at least in part,inhibitory effects on the activity of MMP-2 in posterior sclera. Key words: Experimental myopia ; Defocus ; Sclera ; Matrix metalloproteinase 2 inhibitor ;