Inhibitory effects of odor substances, geosmin and 2-methylisoborneol, on early development of sea urchins

Abstract

Geosmin (1α,10β-dimethyl-9α-decanol) and 2-methylisoborneol ((1- R-exo)-1,2,7,7-tetramethyl-bicyclo-(2,2,1)-heptan-2-ol) (MIB) are volatile terpene derivatives, and have received a great deal of attention because they can cause musty/muddy off-flavor in water and food resources. By the Ames test, these metabolites showed no mutagenicity but antimicrobial activity toward tester strains. While these compounds are produced by various organisms living in aquatic environments, there are few reports of their effects on aquatic organisms. The effects of geosmin and MIB on sea urchin development were examined. The estimated IC 50 (50% inhibitory concentration) values for the formation of the fertilization membrane were 16.67 mg geosmin l −1 and 68.77 mg MIB l −1; those for the cell cleavage were 16.58 mg geosmin l −1 and 66.86 mg MIB l −1, suggesting that the toxicity of geosmin and MIB toward sea urchins are comparable to their toxicity toward Salmonella tester strains in the Ames test. These values are far greater than concentrations of these substances observed in aquatic environments with severe muddy off-flavor problems.