Abstract
Bone‐remodeling imbalance between bone formation by osteoblasts and bone resorption by osteoclasts results in the metabolic bone diseases such as osteoporosis. Gossypin is a naturally‐occurring bioflavonoid seen in vegetables and fruit, as being a treatment for melanoma. The present study investigated that 1‐20 μM gossypin suppressed RANKL‐induced bone resorption in Raw 264.7 macrophages. To differentiate to osteoclasts, macrophages were treated with 50 ng/ml RANKL for 5 days. RANKL enhanced multi‐nucleated osteoclast formation and tartrate‐resistance acid phosphatase activity, which was dose‐dependently attenuated by gossypin. Western blot analysis revealed that gossypin suppressed RANKL induction of integrin β3 and carbonic anhydrase II in macrophage‐derived osteoclasts. These results demonstrate that gossypin retarded the differentiation and activation of osteoclasts to be integrated on bone tissue. Therefore, gossypin is a potent agent in alleviating bone resorption of osteoclasts.Grant Funding Source: Supported by National Research Foundation of Korea (2012012946) and Ministry for Food, Agriculture, Forestry and Fisheries
Published Version
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