Abstract
Aqueous filtrates from membrane filters contain material(s) with UV spectral characteristics quite similar to those of a nonionic isooctyl phenoxy polyethoxy ethanol (IPPE)-type detergent. A permeability test utilizing mammalian cell cultures was employed to evaluate the inhibitory properties of the extract and commercial IPPE. The cell culture test was sensitive to IPPE in concentrations as low as 0.006 percent and produced comparable results with extract concentrations having spectral absorbance (283 mμ) values equal to those of IPPE. Dose-response patterns were markedly similar. Spectral analyses of successive aqueous filtrates were used to study the extractability of the offending agent(s). Treatment of membrane filters with hot water prior to autoclaving rendered the filters safe for sterilization of solutions including cell culture media components.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
