Abstract
The biocompatibility of a wide variety of biomaterials was quantitatively assessed, in a physiologically normal environment" as to cytotoxicity induced in WI-38 cells by cell culture medium extracts. Materials tested included PVC plastic, rubber, silicone rubber, polyethylene, polypropylene, acetal, polyurethane, Teflon, nylon, epoxy, and polystyrene. Cell culture test results were correlated to U.S.P. animal tests. Potential test artifacts, lead, barium, cadmium, and endotoxin were tested for cytotoxicity in WI-38 cells. Cell culture methods yielded more positive tests, particularly rubber, PVC plastic and silicone rubber compounds, than observed in U.S.P. animal tests. Positivity in animal tests did not correlate quantitatively to cytotoxic titers in cell culture. Discrepancies between cell cultures tests and animal tests, specifically rubber compounds, were attributable, in some instances, to differentials in elution efficiency between saline, cottonseed oil, and complete MEM cell culture medium. In other instances, particularly PVC plastics, differences between cell culture and animal test results were due to an inherent difference in the two indicator systems to respond to specific toxic moieties.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Biomaterials, Medical Devices, and Artificial Organs
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.