Inhibitory Effect of Various Breads on DMH-Induced Aberrant Crypt Foci and Colorectal Tumours in Rats

Guangying Qi,Lihua Zhou,Megumi Shobayashi,Kaori Murakami,Tiri Takashima,Fumio Shimamoto,Hiroshi Jikihara,Koji Kakugawa,Sien Zeng,Koichiro Nozoe

doi:10.1155/2015/829096

Abstract

Bread is rich in dietary fibre and many phytochemical compounds, which may influence chemoprevention of colon cancer. In the present study, we evaluated the effect of three kinds of bread on DMH-induced colorectal tumours in F344 rats. F344 rats were divided into four groups (Steinmetz Three-Grain bread, Steinmetz Country bread, White bread, and MF). All groups were injected with 1,2-dimethylhydrazine (DMH, 20 mg/kg body weight) once a week for 8 consecutive weeks from 5 weeks of age. To investigate the antioxidant effect of bread, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging rate of bread and the serum levels of 8-hydroxy-deoxyguanosine (8-OHdG) in rats were examined. The number of colorectal aberrant crypt foci (ACF) and the incidence of colorectal tumours were studied after 34 weeks of DMH treatment. The Steinmetz Three-Grain and Steinmetz Country bread groups had higher scavenging rates of the DPPH free radical and lower serum levels of 8-OHdG and incidence of ACF, adenomas, and adenocarcinomas of colon than the White bread and MF group. Steinmetz Three-Grain bread and Steinmetz Country bread have various ingredient combinations that may inhibit colorectal cancer progression.

Highlights

IntroductionColorectal carcinomas are the second and third most common causes of death of men and women, respectively, in developed countries [1]
Among cancer diseases, colorectal carcinomas are the second and third most common causes of death of men and women, respectively, in developed countries [1]
We have evaluated the effect of two kinds of Steinmetz bread and White bread on DMH-induced colorectal tumours in F344 rats

Summary

Introduction

Colorectal carcinomas are the second and third most common causes of death of men and women, respectively, in developed countries [1]. It has been suggested that diets high in fibre may reduce the risk of incident colorectal cancer (CRC) [4]. Wholegrain cereals are rich in nutrients and many phytochemical compounds, with recognised health benefits, including dietary fibre, a number of phenolic compounds, lignans, vitamins and minerals, and other bioactive components [8]. It remains unclear which components of the diet are the most important, and it is necessary to determine the protective factors. Steinmetz flour may have a health benefit and Steinmetz flour food may reduce the risk of incident colorectal cancer. We have evaluated the effect of two kinds of Steinmetz bread and White bread on DMH-induced colorectal tumours in F344 rats

Materials and Methods

Results

Discussion

Conclusion