Abstract
Objective To study the effect of tumstatin T42 peptide on the induced apoptosis of human hepatocellular carcinoma xenografts in nude mice and the possible mechanism.Methods The human hepatoma cell line HCCLM9 nude model was established.The treatment group and control group (n =10 each) were injected subcutaneously with T42 peptide and saline respectively.The specimens after treatment for two weeks were collected.TdT-mediated dUTP nick end labeling (TUNEL) method was used to detect apoptosis levels,and immunohistochemistry to detect Caspase-3 protein expression.Results The apoptosis index in the T42 peptide treatment group was (8.48 ± 0.58) ％,and that in the control group was (2.56 ± 0.48) ％ (P ＜ 0.05).The expression of Caspase-3 in the T42 treatment group was significantly higher than that in the control group (80％ vs.30％,P ＜ 0.05).After treatment,the tumor weight in the T42 treatment group was (1.83 ±0.22) g and that in the control group was (2.11 ± 1.67) g (P ＜ 0.05).Conclusion T42 peptide can induce apoptosis of hepatoma cells in human hepatocellular carcinoma of nude mice,which may be contributed to the expression of Caspase-3. Key words: Carcinoma, hepatocellular; Tumstatin ; T42 peptide ; Cysteinyl aspartate-specific protease-3
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