Effects of vitamin K2 on the growth and expression of HDGF in transplanted tumor of human liver cancer in nude mice

Abstract

Objective To investigate the effects of vitamin K2 on the growth of transplanted tumor and the expression of HDGF in human hepatocellular carcinoma in nude mice. Methods After the establishment of metastatic human hepatocellular carcinoma models, give the tumor-bearing nude mice oral vitamin K2, the nude mice were randomly divided into three groups, 8 nude mice in each group: control group, vitamin K2 low concentration group [20 mg/(kg·d)], and vitamin K2 high concentration group [45 mg/(kg·d)]. Tumor size, tumor weight were measured during administration, inhibitory rate was calculated. The expression of HDGF protein in transplanted tumor tissues was detected after different concentrations of vitamin K2. Results The inhibitory rate of control group, low vitamin K2 concentration group and high vitamin K2 concentration group was 0%, (36.13±4.38)%, (57.90±2.57)%, respectively. Compared with the control group, the vitamin K2 intervention group showed significantly reduced tumor tissue and increased inhibitory rate (t=25.460, 80.610, P<0.05). Western blotting analysis showed that the grayscale analysis values of HDGF protein expression in low vitamin K2 concentration group and high vitamin K2 concentration group were (0.634±0.030) and (0.278±0.040), which significantly inhibited the expression of HDGF protein in tumor cells of tumor-bearing nude mice, and were statistically significant compared with the control group (t=32.730, 49.520, P<0.05). Conclusion Vitamin K2 inhibited the growth of human hepatocellular carcinoma xenografts in nude mice and down-regulated the expression of HDGF in tumor cells. Key words: Vitamin K2; Nude mice beared human hepatocellular carcinoma; Hepatoma-derived growth factor