Inhibitory Effect of Polypeptide from <em>Argopecten irradias</em> on Mice with Transplanted H22 Hepatocarcinoma and Its Mechanism

Abstract

The objective of this study was to investigate the inhibitory effect of Polypeptide from <em>Argopecten Irradias</em> (PAI) on mice with transplanted H₂₂ hepatocarcinoma. PAI was prepared by enzymatic hydrolysis and separation and the results of its structure identification indicated that it contained five polypeptide components with molecular weights of 700-1000 Da. Forty Kunming mice were randomly divided into H₂₂ hepatocarcinoma group and low, medium and high dose PAI groups. After ig administration of normal saline and different doses of PAI for 10 days, all mice were inoculated with H₂₂ hepatocarcinoma cell suspension to establish models. After continuous gavage for 10 days, the blood was collected from the eyeball and the mice were sacrificed for index detection. It was found that the tumor inhibiting rates of PAI in three dose groups were 28.16, 41.93 and 84.00%, respectively. Compared to H<em>22</em> hepatocarcinoma group, the mutant serum p53, survivin and MDA levels of mice in three PAI-dose groups were significantly reduced (<em>p</em><0.01), the IL-2 and SOD levels were significantly increased (<em>p</em><0.01). The results suggested that PAI had obvious inhibitory effect on mice with H₂₂ hepatocarcinoma cells.