Abstract
Objective To explore the effect of β-catenin inhibitor FH535 on proliferation of HepG2 and its mechanism.Methods HepG2 cells were treated with FH535. Cell proliferation was determined by modified 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay(MTS).cyclin D mRNA expression were determined by real time polymerase chain reaction(RT-PCR)，β-catenin and cyclin D protein expression were determined by western blot.Results Compared with the control group，the proliferation of HepG2 cells was significantly decreased after treatment with FH535,in a dose- and time-dependent manner.cyclin D mRNA and cyclin D protein level were both down-regulated in FH535 group compared with the control group(P<00001),while β-catenin protein expression was not affected.Conclusions FH535 inhibited the proliferation of HepG2 cells.This might through down-regulation of cyclin D mRNA and cyclin D protein.
Published Version
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