Inhibitory effect of chitosan-mediated cytokine response modifier A on interkin-1β and tumor necrosis factor-α expression of chondrocytes

Abstract

Objective To investigate the effects of chitosan/pCDNA3.1 + cytokine rcsponse modifier A (CrmA) nanoparticles on interkin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) expression of chondrocytes.Methods Rabbit chondrocytes were isolated and cultured,and treated with PBS,10 mg/L chitosan (CS) or chitosan/pCDNA3.1 + CrmA nanoparticles,respectively for 6 h.Then 10 μ g/L IL-1β was added into the culture medium.After 48 h,the mRNA expression of IL-13 and TNF-α in chondrocytes was detected by using real-time polymerase chain reaction.Results In chitosan/pCDNA3.1 + CrmA-treated group (0.55 ±0.08),the mRNA expression of IL-1β in chondrocytes was significantly suppressed as compared with corresponding samples of chitosan-treated group (0.69 ± 0.06,P ＜ 0.01) and PBS-treated group (0.99 ±0.04,P ＜ 0.01).There was significant difference in the I L-1 β expression between chitosantreated group and PBS-treated group (P ＜ 0.01).The TNF-α mRNA expression of chondrocytes in chitosan/pCDNA3.1 + CrmA-treated group (0.57 ± 0.07) was lower than that in chitosan-treated group (0.71 ± 0.05,P ＜ 0.01) and PBS-treated group (0.99 ± 0.06,P ＜ 0.01).Significant difference of TNF-α expression between chitoson treated group and PBS treated group was observed (P ＜ 0.01).Conclusion CrmA mediated by chitosan could significantly suppress the mRNA expression of IL-1β and TNF-α of chondrocytes induced bv interkin-1 β.chitosan/pCDNA3.1 * CrmA nanoparticles maybe a potential candidate for therapy of osteoarthritis. Key words: Osteoarthritis; Chondrocyte; Chitosan; Cytokine response modifier A