Abstract
We previously reported that carbachol(CCh) inhibits isoproterenol(ISP)-induced amylase release from isolated rat parotid acinar cells. The present study was undertaken to clarify the mechanism of inhibitory effect of CCh on ISP-induced amylase release. Dispersed acinar cells of rat parotid glands were incubated at 37°C in modified Krebs-Ringer-Tris medium(KRT) for 30min. The following results were obtained: 1) I SP(10-6M)-induced amylase release was inhibited by the pretreatment of CCh(10−6 - 10−4M). 2)The inhibitory effect was blocked by a tropi ne (10”), and also the effect disappeared in Ca-free(1mM EGTA) KRT. 3) ISP(10−6M) - induced increase in cyclic AMP was not affected by CCh(10−5M) in either normal or Ca-free KRT. 4)Ca ionophore A231 87(10-5M) markedly inhibited the ISP-induced amy lase release. 5)Both CCh(10-5M) and ISP(10-6M) increased 45Ca uptake and its efflux. CCh significantly enhanced the effect of ISP on 45ca uptake. These results suggest that Ca2+ influx may play an important role in the inhibitory effect of CCh on ISP-induced amylase release from isolated rat parotid acinar cells.
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