Abstract
This study investigates the inhibitory effect of Brazilin on the melanogenesis in B16/F10 melanoma. Cell viability was measured using the MTT. The reagent concentrations were 5, 10, 20, 40, and 60 mg/ml. Melanoma cells were cultured for 72 hours with Brazilin treated at different concentrations. Total melanin content and tyrosinase activity in melanoma cells were evaluated. Alpha melanocyte stimulating hormone(α-MSH) was used to activate the melanoma cells. Viability of melanoma cells did not significantly decrease in 5, 10, 20, and 40 mg/ml Brazilin compared to the control group. Cell shapes did not change in any group. Total melanin content in α-MSH-stimulated melanoma cells was significantly inhibited in the 40 mg/ml Brazilin group compared to the control group. Tyrosinase α-MSH stimulation of melanoma cells was significantly reduced in the 40 mg/ml Brazilin group compared to the control group. According to the results, Brazilin may have an ability to suppress total melanin and tyrosinase.
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