Inhibitory Effect of Aqueous Extract of Moringa oleifera and Newbuoldia laevis Leaves on Ferrous Sulphate and Sodium Nitroprusside Induced Oxidative Stress in Rat’s Testes in Vitro

Seun F Akomolafe,Afolabi A Akindahunsi,Olusola Adeyanju,Ayodele J Akinyemi,Ganiyu Oboh

doi:10.4236/ojmc.2012.24015

Abstract

Oxidative stress has been identified as one of the factors that affects fertility status. Therefore, this study sought to investigate the inhibitory effect of aqueous extract of Moringa oleifera and Newbuoldia laevis leaves on FeSO4 and Sodium Nitroprusside (SNP) induced lipid peroxidation in rat testes in vitro. Incubation of the testes tissue homogenate in the presence of FeSO4 and SNP caused a significant increase in the malondialdehyde (MDA) contents of the testes. The aqueous extract from both Moringa oleifera and Newbuoldia laevis leaves caused a significant decrease in the MDA contents of the testes in a dose-dependent manner. However, aqueous extract from Moringa oleifera leaf (EC50 = 0.29 mg/ml) had a significant (P2+ induced lipid peroxidation in the rat testes homogenate than that of Newbuoldia laevis leaf extract (EC50 = 0.58 mg/ml); while there was no significant (P2+ chelating and reducing power. Therefore, these plants have potential to prevent oxidative stress in testes and improve fertility outcomes.

Highlights

Infertility is a major clinical problem, affecting people medically and psychosocially
This study sought to investigate the inhibitory effect of aqueous extract of Moringa oleifera and Newbuoldia laevis leaves on FeSO4 and Sodium Nitroprusside (SNP) induced lipid peroxidation in rat testes in vitro
The aqueous extract from both Moringa oleifera and Newbuoldia laevis leaves caused a significant decrease in the MDA contents of the testes in a dose-dependent manner

Summary

Introduction

Infertility is a major clinical problem, affecting people medically and psychosocially. Published literature data indicates that 15% of all couples in the United States are infertile, and the male factor is responsible for 25% of these cases [1]. In many cases the cause of male infertility, oxidative stress (OS) has been identified as one of the major factors that affects fertility status and has been extensively studied in recent years. Breakdown products of oxygen such as ROS can be detrimental to cell function and survival [4]. Examples of ROS include the hydroxyl superoxide radical, hydrogen peroxide, peroxyl radical, and hypochlorite ion. These are the common forms of ROS that have been considered injurious to sperm survival and function when present in abundance

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