Inhibitors of protein glycosylation inhibit the differentiation of Friend erythroleukemia cells

Abstract

Tunicamycin, 2-deoxy- d-glucose and 2-deoxy-2-fluoro- d-glucose inhibit dimethyl sulfoxide-induced differentiation of Friend cells. This inhibition, characterized by inhibition of hemoglobin synthesis, is accompanied by a specific inhibition of protein glycosylation. The results of cloning experiments indicate that this inhibition specifically affects cells in the period preceding their commitment. These results suggest that glycoprotein synthesis is a requirement for Friend erythroleukemia cells in order to initiate the expression of the terminal differentiation program.