Inhibitive efficacy of Nymphoides indica rhizome extract on α-glucosidase, and cross-link formation of advanced glycation end product.

Abstract

To investigate the inhibitive efficacy of Nymphoides Indica (L.) Kuntze rhizome extract on α-glucosidase and on cross-link formation of advanced glycation end products (AGEs). The plant extracts were prepared by cold maceration and fractionated in solvents of diverse polarity. The in vitro α-glucosidase inhibition assay, fluorescence spectrometry and SDS-PAGE analysis was performed for antiglycation assays. During α-glucosidase inhibition assay significant inhibition by chloroform (0.43 mg/mL) and methanol fractions (0.66 mg/mL) was noticed. During the AGEs inhibition assay, both oxidative (BSA-MGO) and non-oxidative (BSA-glucose) modes were employed. The inhibition of AGEs by total extract was considered moderate (IC50 0.10 mg/mL) as a result of non-oxidative mode, whereas in case of oxidative mode (BASA-MGO) no activity was recorded. Among fractions the methanolic fraction presented significant results both in oxidative (IC50 0.01 mg/mL) and non-oxidative modes (IC50 0.3 mg/mL). Likewise the ethyl acetate fraction was more active in non-oxidative mode (IC50 0.04 mg/mL) compared to oxidative mode (IC50 0.32 mg/mL). During assay for inhibition of cross-link formation, the chloroform fraction significantly inhibited cross-link formation in a dose dependent mode. It was finally concluded that N. Indica rhizome extract possesses significant properties that inhibit α-glucosidase, and AGEs cross-link formation.