Inhibition of Oral Bacterial Growth (Streptococcus Mutans and Porphyromonas Gingivalis) using Black Rice Bran Ethanol Extract (Oryza Sativa L.) as A Natural Mouthwash

Abstract

Oral health problems in Indonesia are still relatively high. Indonesian National Health Research 2018 stated that oral health problem’s proportion in Indonesia is 57.6%. Diseases in the oral cavity occur due to accumulation of bacteria, including bacteria that cause dental caries (Streptococcus mutans) and periodontal disease (Porphyromonas gingivalis). Current scientific methods prioritize prevention care to take care of oral health such as teeth brushing regularly using toothpaste and using mouthwash rather than surgical intervention. However, mouthwash contains alcohol, in which can increase the risk of developing oral cancer. Rice bran is a natural comestible containing antibacterial substances. Differences in pigment in rice affect its antibacterial activities. Hence, this research novelty lies in using black rice bran extract (Oryza sativa L.) formulated mouthwash. This study was a laboratory experimental study with post-test only with control group design. The study itself was divided into several phases, extract making to inhibition test. Statistical analysis resulted bran extract with concentration of 10, 20, 40, 80, and 100% with ampicillin positive control each have different inhibition zone and are statistically significant (p<0.05) to Streptococcus mutans (p=0.00) and Porphyromonas gingivalis (p=0.00). Based on study conducted mouthwash A and negative control was used as comparative variables for mouthwash with 10% concentration and shows significance level of 0.00 (p<0.05) which means there is a significant difference between each group. Black Rice Bran extract mouthwash as inhibitor Streptococcus mutans and Porphyromonas gingivalis growth bacteria.   How to Cite this Article Pubmed Style Marhamah, Harun Achmad, Hendrastuti Handayani, Sherly Horax, Fajriani, Nurhaedah H. Galib B, Sasmita M. Arief. of Oral Bacterial Growth (Streptococcus Mutans and Porphyromonas Gingivalis) using Black Rice Bran Ethanol Extract (Oryza Sativa L.) as A Natural Mouthwash . SRP. 2020; 11(5): 666-671. doi:10.31838/srp.2020.5.93 Web Style Marhamah, Harun Achmad, Hendrastuti Handayani, Sherly Horax, Fajriani, Nurhaedah H. Galib B, Sasmita M. Arief. of Oral Bacterial Growth (Streptococcus Mutans and Porphyromonas Gingivalis) using Black Rice Bran Ethanol Extract (Oryza Sativa L.) as A Natural Mouthwash . http://www.sysrevpharm.org/?mno=114289 [Access: March 30, 2021]. doi:10.31838/srp.2020.5.93 AMA (American Medical Association) Style Marhamah, Harun Achmad, Hendrastuti Handayani, Sherly Horax, Fajriani, Nurhaedah H. Galib B, Sasmita M. Arief. of Oral Bacterial Growth (Streptococcus Mutans and Porphyromonas Gingivalis) using Black Rice Bran Ethanol Extract (Oryza Sativa L.) as A Natural Mouthwash . SRP. 2020; 11(5): 666-671. doi:10.31838/srp.2020.5.93 Vancouver/ICMJE Style Marhamah, Harun Achmad, Hendrastuti Handayani, Sherly Horax, Fajriani, Nurhaedah H. Galib B, Sasmita M. Arief. of Oral Bacterial Growth (Streptococcus Mutans and Porphyromonas Gingivalis) using Black Rice Bran Ethanol Extract (Oryza Sativa L.) as A Natural Mouthwash . SRP. (2020), [cited March 30, 2021]; 11(5): 666-671. doi:10.31838/srp.2020.5.93 Harvard Style Marhamah, Harun Achmad, Hendrastuti Handayani, Sherly Horax, Fajriani, Nurhaedah H. Galib B, Sasmita M. Arief (2020) of Oral Bacterial Growth (Streptococcus Mutans and Porphyromonas Gingivalis) using Black Rice Bran Ethanol Extract (Oryza Sativa L.) as A Natural Mouthwash . SRP, 11 (5), 666-671. doi:10.31838/srp.2020.5.93 Turabian Style Marhamah, Harun Achmad, Hendrastuti Handayani, Sherly Horax, Fajriani, Nurhaedah H. Galib B, Sasmita M. Arief. 2020. of Oral Bacterial Growth (Streptococcus Mutans and Porphyromonas Gingivalis) using Black Rice Bran Ethanol Extract (Oryza Sativa L.) as A Natural Mouthwash Reviews in Pharmacy, 11 (5), 666-671. doi:10.31838/srp.2020.5.93 Chicago Style Marhamah, Harun Achmad, Hendrastuti Handayani, Sherly Horax, Fajriani, Nurhaedah H. Galib B, Sasmita M. Arief. Inhibition of Oral Bacterial Growth (Streptococcus Mutans and Porphyromonas Gingivalis) using Black Rice Bran Ethanol Extract (Oryza Sativa L.) as A Natural Mouthwash . Systematic Reviews in Pharmacy 11 (2020), 666-671. doi:10.31838/srp.2020.5.93 MLA (The Modern Language Association) Style Marhamah, Harun Achmad, Hendrastuti Handayani, Sherly Horax, Fajriani, Nurhaedah H. Galib B, Sasmita M. Arief. Inhibition of Oral Bacterial Growth (Streptococcus Mutans and Porphyromonas Gingivalis) using Black Rice Bran Ethanol Extract (Oryza Sativa L.) as A Natural Mouthwash . Systematic Reviews in Pharmacy 11.5 (2020), 666-671. Print. doi:10.31838/srp.2020.5.93 APA (American Psychological Association) Style Marhamah, Harun Achmad, Hendrastuti Handayani, Sherly Horax, Fajriani, Nurhaedah H. Galib B, Sasmita M. Arief (2020) of Oral Bacterial Growth (Streptococcus Mutans and Porphyromonas Gingivalis) using Black Rice Bran Ethanol Extract (Oryza Sativa L.) as A Natural Mouthwash Reviews in Pharmacy, 11 (5), 666-671. doi:10.31838/srp.2020.5.93