Abstract
Shewanella putrefaciens (S. putrefaciens) is a major spoilage bacterium in aquatic products and has strong capacity to form biofilm, which contributes to its contamination and persistence in food system. The study aimed to investigate the effect of laurel essential oil (LEO) on biofilm formation of S. putrefaciens by phenotypic analysis and explore its potential mechanisms using RNA sequencing (RNA-seq) analysis. LEO significantly reduced biofilm formation in laboratory media and food broths (oyster juice and sea bass juice), as well as on shrimp surfaces and different food contact surfaces (304 type stainless steel, glass and food-grade silicone). Moreover, LEO also inhibited bacterial motility, decreased extracellular polymeric substances (EPS) contents, and modified biofilm structure. Additionally, RNA-seq analysis identified 229 differentially expressed genes (DEGs), which were involved in transcriptional regulation, multidrug efflux pump transporter, ribosome, cell motility, environmental factors and two-component systems. In conclusion, these findings identify LEO as a potential natural antibiofilm agent for S. putrefaciens and provide insights into its anti-biofilm mechanisms.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.